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Anazasi Pulse Programs

Since we purchased the Anazasi EFT-90 upgrade in January of 2000, we have developed a number of experiments that expand the original capabilities of the instrument, and allow us not only to perform better routine analyses, but to include advanced topics in our courses which cover NMR spectroscopy. Here are copies of the pulse sequences we developed at USP. Most of them have extensive explanations through the pulse program and are simple to understand. If you have any questions or suggestions, let us know.

Many of the pulse programs require their own initialization (".ini") file to work properly. Usually, this just sets the correct number of scans for the phase cycle to operate properly, spectral width, and decoupler power values. Also, the pulse sequences using DANTE pulse trains and the TOCSY sequences require additional pulses defined in the "pulses.txt" file. e-mail the authors for instructions on how to set these properly.

Carbon Pulse Sequences

inept.ppg The regular INEPT pulse sequence with no refocusing. Includes block accumulation.

ineptplus.ppg A variation of the INEPT experiment. Its advantage is that there is no distortion of the multiplet's intensities. Allows for block accumulation.

pendant.ppg PENDANT pulse sequence. Similar to Refocused INEPT, but quaternary carbons also show up with the same sign than methylenes. Allows for block accumulation.

deptbapr.ppg This is the classical DEPT pulse sequence, modified from the original Anazasi pulse program so that the user can select between a DEPT-45, 90, or 135, and allows for block accumulation.

seft.ppg Spin-Echo Fourier Transform pulse sequence. Similar to the APT pulse sequence, but uses a longer excitation pulse which means extended repetition rates. Allows for block accumulation.

invrecbapr.ppg The Inversion Recovery pulse sequence for the measurement of T1 relaxation in carbon. Modified from the original Anazasi invrec pulse program to include block accumulation.

inadequate.ppg A very experimental 1D-INADEQUATE pulse sequence. We beleive that the pulse program is fine, but haven't found a suitable sample yet.

coloc.ppg The COLOC pulse sequence for detection of long-range 1H-13C connectivities. The pulse sequence allows the user to include a BIRD filter to eliminate one-bond correlations. This one has been extensivelly tested and works very well.

hetero2dj.ppg The classic Heteronuclear 2D J-Spectroscopy experiment. Gives 13C chemical shifts on the F2 axis and 13C-1H J-couplings on the F1 axis. Works well with concentrated samples.

Proton Pulse Sequences

jar.ppg The classical Jump-And-Return pulse sequence for elimination of solvent lines. The user can select between a 1:1, a 1:2:1, or a 1:3:3:1 binomial pulse. Phase artifacts can be removed with the utilities provided in EFTtools (see below).

invsup.ppg The typical solvent supression experiment by Inversion Recovery. Nice to compare results of jar.ppg and selsup.ppg (see below) to this pulse sequence.

cosyph.ppg Variation of the COSY pulse sequence provided with the EFT-90 that has an eight-step phase cycle. Can be processed in magnitude or phase-sensitive mode.

ctcosy.ppg Constant-Time COSY pulse sequence. Uses a spin-echo in a constant-time evolution to 'decouple' the signal in F1. The constant-time can be modified to maximize the polarization trasfer efficiency of a coupled signal of interest.

dqfcosy.ppg Double-Quantum Filtered COSY pulse sequence. Has an eight-step phase cycle that filters out single quantum coherence, giving cleaner diagonals and less artifacts in samples with singlets. Try it on ethyl acetate.

homo2dj.ppg The classical Homonuclear 2D J-Spectroscopy experiment. Gives 1H chemical shift on the F2 axis and 1H-1H J-coupling on the F1 axis. Works well except for samples with strong second-order couplings.

calib90.ppg A simple routine that can be used to calibrate the transmiter or decoupler 90 degree pulses. Gives you a series of experiments at different PW values that can be analyzed easily using NUTS.

shape.ppg This pulse sequence tests for the selectivity of shaped pulses made with PulseShaper (see above). It creates the shape by reading the pulse profile and transforming it into a pulsewidth modulated DANTE train. The offest is swept to estimate the selectivity of the shaped pulse. It can be used as a block in other pulse sequences. Needless to say, the pulse program is pretty involved.

dante.ppg Selective excitation with a regular DANTE pulse train. The user can select all the parameters of the DANTE train, and the offest is swept to estimate selectivity. As opposed to shape.ppg, the hardware loop is used to create the DANTE train.

tocsy.ppg This is our TOtal Correlation SpectroscopY pulse program, and another very elaborate one indeed. It uses the decoupler at low power to create a MLEV-17 spin-lock, including trimming pulses. The pulse program is still in the works and requires some experimentation.

inept.ppg	The regular INEPT pulse sequence with no refocusing. Includes block accumulation.
ineptplus.ppg	A variation of the INEPT experiment. Its advantage is that there is no distortion of the multiplet's intensities. Allows for block accumulation.
pendant.ppg	PENDANT pulse sequence. Similar to Refocused INEPT, but quaternary carbons also show up with the same sign than methylenes. Allows for block accumulation.
deptbapr.ppg	This is the classical DEPT pulse sequence, modified from the original Anazasi pulse program so that the user can select between a DEPT-45, 90, or 135, and allows for block accumulation.
seft.ppg	Spin-Echo Fourier Transform pulse sequence. Similar to the APT pulse sequence, but uses a longer excitation pulse which means extended repetition rates. Allows for block accumulation.
invrecbapr.ppg	The Inversion Recovery pulse sequence for the measurement of T1 relaxation in carbon. Modified from the original Anazasi invrec pulse program to include block accumulation.
inadequate.ppg	A very experimental 1D-INADEQUATE pulse sequence. We beleive that the pulse program is fine, but haven't found a suitable sample yet.
coloc.ppg	The COLOC pulse sequence for detection of long-range 1H-13C connectivities. The pulse sequence allows the user to include a BIRD filter to eliminate one-bond correlations. This one has been extensivelly tested and works very well.
hetero2dj.ppg	The classic Heteronuclear 2D J-Spectroscopy experiment. Gives 13C chemical shifts on the F2 axis and 13C-1H J-couplings on the F1 axis. Works well with concentrated samples.

jar.ppg	The classical Jump-And-Return pulse sequence for elimination of solvent lines. The user can select between a 1:1, a 1:2:1, or a 1:3:3:1 binomial pulse. Phase artifacts can be removed with the utilities provided in EFTtools (see below).
invsup.ppg	The typical solvent supression experiment by Inversion Recovery. Nice to compare results of jar.ppg and selsup.ppg (see below) to this pulse sequence.
cosyph.ppg	Variation of the COSY pulse sequence provided with the EFT-90 that has an eight-step phase cycle. Can be processed in magnitude or phase-sensitive mode.
ctcosy.ppg	Constant-Time COSY pulse sequence. Uses a spin-echo in a constant-time evolution to 'decouple' the signal in F1. The constant-time can be modified to maximize the polarization trasfer efficiency of a coupled signal of interest.
dqfcosy.ppg	Double-Quantum Filtered COSY pulse sequence. Has an eight-step phase cycle that filters out single quantum coherence, giving cleaner diagonals and less artifacts in samples with singlets. Try it on ethyl acetate.
homo2dj.ppg	The classical Homonuclear 2D J-Spectroscopy experiment. Gives 1H chemical shift on the F2 axis and 1H-1H J-coupling on the F1 axis. Works well except for samples with strong second-order couplings.
calib90.ppg	A simple routine that can be used to calibrate the transmiter or decoupler 90 degree pulses. Gives you a series of experiments at different PW values that can be analyzed easily using NUTS.
shape.ppg	This pulse sequence tests for the selectivity of shaped pulses made with PulseShaper (see above). It creates the shape by reading the pulse profile and transforming it into a pulsewidth modulated DANTE train. The offest is swept to estimate the selectivity of the shaped pulse. It can be used as a block in other pulse sequences. Needless to say, the pulse program is pretty involved.
dante.ppg	Selective excitation with a regular DANTE pulse train. The user can select all the parameters of the DANTE train, and the offest is swept to estimate selectivity. As opposed to shape.ppg, the hardware loop is used to create the DANTE train.
tocsy.ppg	This is our TOtal Correlation SpectroscopY pulse program, and another very elaborate one indeed. It uses the decoupler at low power to create a MLEV-17 spin-lock, including trimming pulses. The pulse program is still in the works and requires some experimentation.